5(6)-TAMRA

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Chemical Structure| 98181-63-6 同义名 : 5(6)-Carboxytetramethylrhodamine
CAS号 : 98181-63-6
货号 : A559208
分子式 : C25H22N2O5
纯度 : 98% (mix)
分子量 : 430.45
MDL号 : MFCD00467499
存储条件：

Pure form Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 20 mg/mL(46.46 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 5(6)-TAMRA contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. It is a bright, orange-fluorescent dye that produces conjugates with absorption/emission maxima of ~555/580 nm. Conjugates of the dyes with "small molecules" provided specific labeling (covalent and non-covalent) of organelles as well as of components of the cytoskeleton in living cells and were combined with fluorescent probes prepared from 610CP and SiR dyes and applied in two-color STED microscopy with a 775 nm STED laser[3]. Having combined TAMRA-labeled DNA probe and the power of RNA-seq technology, TAMRA+ cells displayed gene expression signatures characteristic of both stem cells and cancer cells[4]. Co-internalization of the orexin-1 receptor was observed by monitoring the binding and trafficking of TAMRA-(5- and 6-carboxytetramethylrhodamine) labelled orexin-A[5]. TAMRA-IL-13-f-Gd3N@C80 (attached a TAMRA fluorescent label and conjugated the IL-13 peptide) has been studied for MRI of IL-13Rα2 expression on glioma tumors in mice[6].
临床研究
NCT号 适应症或疾病 临床期 招募状态 预计完成时间 地点
NCT02339740 Acute Promyelocytic Leukemia W... 展开 >>ith PML-RARA 收起 << Phase 3 Recruiting - -
NCT03007147 B Acute Lymphoblastic Leukemia... 展开 >> BCR-ABL1 Fusion Protein Expression Minimal Residual Disease Philadelphia Chromosome Positive T Acute Lymphoblastic Leukemia Untreated Adult Acute Lymphoblastic Leukemia Untreated Childhood Acute Lymphoblastic Leukemia 收起 << Phase 3 Recruiting June 30, 2026 -
NCT02101853 B Acute Lymphoblastic Leukemia Phase 3 Recruiting - -
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.32mL

0.46mL

0.23mL

11.62mL

2.32mL

1.16mL

23.23mL

4.65mL

2.32mL
参考文献

[1]Fuchs SM, Raines RT. Pathway for polyarginine entry into mammalian cells. Biochemistry. 2004 Mar 9;43(9):2438-44.

[2]Gerber D, Shai Y. Insertion and organization within membranes of the delta-endotoxin pore-forming domain, helix 4-loop-helix 5, and inhibition of its activity by a mutant helix 4 peptide. J Biol Chem. 2000 Aug 4;275(31):23602-7.

[3]Grimm F, Rehman J, Stoldt S, Khan TA, Schlötel JG, Nizamov S, John M, Belov VN, Hell SW. Rhodamines with a Chloronicotinic Acid Fragment for Live Cell Superresolution STED Microscopy*. Chemistry. 2021 Apr 1;27(19):6070-6076

[4]Potter EA, Dolgova EV, Proskurina AS, Efremov YR, Minkevich AM, Rozanov AS, Peltek SE, Nikolin VP, Popova NA, Seledtsov IA, Molodtsov VV, Zavyalov EL, Taranov OS, Baiborodin SI, Ostanin AA, Chernykh ER, Kolchanov NA, Bogachev SS. Gene expression profiling of tumor-initiating stem cells from mouse Krebs-2 carcinoma using a novel marker of poorly differentiated cells. Oncotarget. 2017 Feb 7;8(6):9425-9441

[5]Evans NA, Groarke DA, Warrack J, Greenwood CJ, Dodgson K, Milligan G, Wilson S. Visualizing differences in ligand-induced beta-arrestin-GFP interactions and trafficking between three recently characterized G protein-coupled receptors. J Neurochem. 2001 Apr;77(2):476-85

[6]Leung K. TAMRA-IL-13-Conjugated functionalized gadolinium metallofullerene (Gd3N@C80(OH)-26(CH2CH2COOH)-16). 2011 Sep 4 [updated 2011 Nov 17]. In: Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2004–2013