Hoechst 33342 3HCl

产品说明书

Print
Chemical Structure| 875756-97-1 同义名 : bisBenzimide H 33342 trihydrochloride; HOE 33342 trihydrochloride; NSC 334072; HOE 33342; Bisbenzimide; Hoechst 33342 (hydrochloride); Hoechst 33342 trihydrochloride
CAS号 : 875756-97-1
货号 : A416033
分子式 : C27H31Cl3N6O
纯度 : 98%
分子量 : 561.93
MDL号 : MFCD30179380
存储条件：

Pure form Inert atmosphere, store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 45 mg/mL(80.08 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 5.5 mg/mL(9.79 mM)
生物活性
描述 Hoechst 33342 is a nuclear marker dye in Hoechst series, primarily binding to the A/T-rich regions of DNA strands. Its binding affinity is not restricted to DNA, but the fluorescence intensity is significantly enhanced when bound to A/T-rich double-stranded DNA, making it a valuable tool for live cell labeling. The fluorescence intensity of Hoechst dye increases with the solution's pH, enabling its application across a range of biological research fields[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.78mL

0.36mL

0.18mL

8.90mL

1.78mL

0.89mL

17.80mL

3.56mL

1.78mL
参考文献

[1]Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.