BODIPY 493/503

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Chemical Structure| 121207-31-6 同义名 : Pyrromethene 546; BDP 493/503 lipid stain; Bodipy495
CAS号 : 121207-31-6
货号 : A411914
分子式 : C14H17BF2N2
纯度 : 99%
分子量 : 262.11
MDL号 : MFCD00467372
存储条件：

Pure form Keep in dark place, inert atmosphere, room temperature

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 4 mg/mL(15.26 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 BODIPY 493/503 (BODIPY) is an alternative lipid dye with lower background staining and narrower emission spectra[2]. BODIPY 493/503 was a convenient and simple approach to visualize lipid droplets (LDs) in both sectioned skeletal muscle and cultured adult single fibers. Furthermore, the dye was effective in both fixed and nonfixed cells, and the staining seemed unaffected by permeabilization[3]. The use of the fluorescent neutral lipid dye 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503) to facilitate quantification of neutral lipid content by flow cytometry and observation of LDs (Lipid droplets) by microscopy[4]. BODIPY 493/503 contains a nonpolar structure that, upon binding to neutral lipid, emits a green fluorescence signal with a narrow wavelength range, making it an ideal fluorophore for multi-labeling experiments[5]. BODIPY 493/503 is well suited for quantitative analysis of LDs for high-throughput screening (HTS) applications[6]. BODIPY 493/503 produced an overall better staining for PHB (Poly [(R)-3-hydroxybutyric acid) than did Nile red[7].
Protocol from BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometryde novo lipogenesis[8]
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.82mL

0.76mL

0.38mL

19.08mL

3.82mL

1.91mL

38.15mL

7.63mL

3.82mL
参考文献

[1]Qiu B, Simon MC. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016;6(17):e1912. doi:10.21769/BioProtoc.1912

[2]Strauss JA, Shepherd DA, Macey M, Jevons EFP, Shepherd SO. Divergence exists in the subcellular distribution of intramuscular triglyceride in human skeletal muscle dependent on the choice of lipid dye. Histochem Cell Biol. 2020 Oct;154(4):369-382

[3]Spangenburg EE, Pratt SJP, Wohlers LM, Lovering RM. Use of BODIPY (493/503) to visualize intramuscular lipid droplets in skeletal muscle. J Biomed Biotechnol. 2011;2011:598358

[4]Qiu B, Simon MC. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016 Sep 5;6(17):e1912

[5]Baumann JM, Kokabee L, Wang X, Sun Y, Wong J, Conklin DS. Metabolic Assays for Detection of Neutral Fat Stores. Bio Protoc. 2015 Jun 20;5(12):e1511

[6]Ranall MV, Gabrielli BG, Gonda TJ. High-content imaging of neutral lipid droplets with 1,6-diphenylhexatriene. Biotechniques. 2011 Jul;51(1):35-6, 38-42

[7]Kacmar J, Carlson R, Balogh SJ, Srienc F. Staining and quantification of poly-3-hydroxybutyrate in Saccharomyces cerevisiae and Cupriavidus necator cell populations using automated flow cytometry. Cytometry A. 2006 Jan;69(1):27-35

[8]BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry