6-CFDA

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Chemical Structure| 3348-03-6 同义名 : 6-羧基荧光素二乙酸酯 ;6-Carboxyfluorescein diacetate
CAS号 : 3348-03-6
货号 : A383451
分子式 : C25H16O9
纯度 : 95% HPLC
分子量 : 460.39
MDL号 : MFCD00036871
存储条件:

Pure form Sealed in dry, store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月

溶解度 :

DMSO: 105 mg/mL(228.07 mM),配合低频超声助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO

动物实验配方:
生物活性
描述 6-CFDA, recognized as an aliphatic luciferin-like entity, permeates cells freely, converting into carboxyl fluorescein (CF) via intracellular non-specific lipase activity. This transformation endows CF with an extra negative charge, enhancing its cellular retention relative to other fluorescein dyes[1][2][3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.17mL

0.43mL

0.22mL

10.86mL

2.17mL

1.09mL

21.72mL

4.34mL

2.17mL

参考文献

[1]Yang T, et al. A novel nonradioactive CFDA assay to monitor the cellular immune response in myeloid leukemia. Immunobiology. 2013 Apr;218(4):548-53.

[2]Card SD, et al. Assessment of fluorescein-based fluorescent dyes for tracing Neotyphodium endophytes in planta. Mycologia. 2013 Jan-Feb;105(1):221-9.

[3]Fang X, et al. Bone marrow-derived endothelial progenitor cells are involved in aneurysm repair in rabbits. J Clin Neurosci. 2012 Sep;19(9):1283-6.