Oligomycin A

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Chemical Structure| 579-13-5 同义名 : MCH 32
CAS号 : 579-13-5
货号 : A358360
分子式 : C45H74O11
纯度 : 99%
分子量 : 791.06
MDL号 : MFCD00065705
存储条件：

Pure form Sealed in dry, store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(132.73 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

无水乙醇: 100 mg/mL(126.41 mM)，配合低频超声，并水浴加热至45℃助溶，注意：无水乙醇开封后，易挥发，也会吸收空气中的水分，导致溶解能力下降，请避免使用开封较久的乙醇
生物活性
靶点

  • ATPase
描述 Oligomycin is a macrolide antibiotic, usually used as a potent inhibitor of mitochondrial F0F1-ATPase and oxidative phosphorylation, with Ki value of 1μM for inhibition of yeast intact mitochondrial ATPase activity and 0.1μM for Triton X-100-solubilized mitochondria[1]. Oligomycin at concentration of 1.5-3μM stimulated lysosome acidification due to shunting of membrane potential[2]. Treatment with 2.5μM oligomycin dramatically reduced ATP level in MII oocytes exposure to H2O2[3]. Oligomycin A exhibited antiproliferative effect on the JARID1B high subpopulation of WM3734 cells at concentration ranging in 0.1-10μg/ml post 24-72h due to the inhibition of mitochondrial respiration[4].
作用机制 Oligomycin could bind to the c10 ring of the of the yeast mitochondrial ATP synthase thereby making contact with two neighboring molecules at a position. The carboxyl side chain of Glu59, which is essential for proton translocation, forms an H-bond with oligomycin.[5]
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.26mL

0.25mL

0.13mL

6.32mL

1.26mL

0.63mL

12.64mL

2.53mL

1.26mL
参考文献

[1]Salomon AR, Voehringer DW, et al. Apoptolidin, a selective cytotoxic agent, is an inhibitor of F0F1-ATPase. Chem Biol. 2001 Jan;8(1):71-80.

[2]Van Dyke RW, et al. Acidification of rat liver lysosomes: quantitation and comparison with endosomes. Am J Physiol. 1993 Oct;265(4 Pt 1):C901-17.

[3]Zhang X, Wu XQ, et al. Deficit of mitochondria-derived ATP during oxidative stress impairs mouse MII oocyte spindles. Cell Res. 2006 Oct;16(10):841-50. Epub 2006 Sep 19.

[4]Roesch A, Vultur A, et al. Overcoming intrinsic multidrug resistance in melanoma by blocking the mitochondrial respiratory chain of slow-cycling JARID1B(high) cells. Cancer Cell. 2013 Jun 10;23(6):811-25.

[5]Symersky J, Osowski D, et al. Oligomycin frames a common drug-binding site in the ATP synthase. Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):13961-5.