5-Carboxyfluorescein diacetate N-succinimidyl ester

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Chemical Structure| 150206-05-6 同义名 : -
CAS号 : 150206-05-6
货号 : A319961
分子式 : C29H19NO11
纯度 : 98%
分子量 : 557.46
MDL号 : MFCD00037466
存储条件：

Pure form Inert atmosphere, store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 20 mg/mL(35.88 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell-permeable dye (Excitation=492 nm, Emission=517 nm) that labels cells by covalently binding to intracellular molecules. It is used for tracking lymphocyte migration and proliferation[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.79mL

0.36mL

0.18mL

8.97mL

1.79mL

0.90mL

17.94mL

3.59mL

1.79mL
参考文献

[1]Christopher R Parish, et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Curr Protoc Immunol. 2009 Feb;Chapter 4:Unit4.9.