Obacunone

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Chemical Structure| 751-03-1 同义名 : AI3-37934; CCRIS 8657
CAS号 : 751-03-1
货号 : A315545
分子式 : C26H30O7
纯度 : 98%
分子量 : 454.51
MDL号 : MFCD00075888
存储条件：

Pure form Sealed in dry, 2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 250 mg/mL(550.04 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
生物活性
靶点

  • Aromatase

    Aromatase, IC50:28.4 μM
描述 Obacunone belongs to a class of unique triterpenoids called limonoids, present in Citrus species. Obacunone possesses antivirulence activity and demonstrates inhibition of cell-cell signaling in Vibrio harveyi and Escherichia coli O157:H7[3]. Obacunone inhibited SW480 cell proliferation with IC50 values of 97 μM at 24h. Sequence of events such as decreased ratio of bcl2/bax gene transcription, activation of caspase-3, fragmentation of DNA in cells treated with obacunone demonstrated induction of apoptosis by limonoids[4]. Obacunone stimulated the transcriptional activity of the bile acids-specific G protein-coupled receptor, TGR5, in a dose-dependent manner. In addition, obacunone inhibited adipocyte differentiation in 3T3-L1 cells and antagonized ligand-stimulated peroxisome proliferator-activated receptor γ (PPARγ) transcriptional activity[5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.20mL

0.44mL

0.22mL

11.00mL

2.20mL

1.10mL

22.00mL

4.40mL

2.20mL
参考文献

[1]Murthy KN, Jayaprakasha GK, Patil BS. Cytotoxicity of obacunone and obacunone glucoside in human prostate cancer cells involves Akt-mediated programmed cell death. Toxicology. 2015 Mar 2;329:88-97.

[2]Kim J, Jayaprakasha GK, Patil BS. Obacunone exhibits anti-proliferative and anti-aromatase activity in vitro by inhibiting the p38 MAPK signaling pathway in MCF-7 human breast adenocarcinoma cells. Biochimie. 2014 Oct;105:36-44.

[3]Vikram A, Jayaprakasha GK, Jesudhasan PR, Pillai SD, Patil BS. Obacunone represses Salmonella pathogenicity islands 1 and 2 in an envZ-dependent fashion. Appl Environ Microbiol. 2012;78(19):7012-7022

[4]Chidambara Murthy KN, Jayaprakasha GK, Patil BS. Obacunone and obacunone glucoside inhibit human colon cancer (SW480) cells by the induction of apoptosis. Food Chem Toxicol. 2011;49(7):1616-1625

[5]Horiba T, Katsukawa M, Mita M, Sato R. Dietary obacunone supplementation stimulates muscle hypertrophy, and suppresses hyperglycemia and obesity through the TGR5 and PPARγ pathway. Biochem Biophys Res Commun. 2015;463(4):846-852