Human KARS (C-6His, HEK293)
产品说明书
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同义名 : | 重组人KARS蛋白(C-6His, HEK293) ;Recombinant Human KARS (C-6His, HEK293); Lysine--tRNA Ligase; Lysyl-tRNA Synthetase; LysRS; KARS; KIAA0070 |
| CAS号 : | N/A | |
| 货号 : | A2925923 | |
| 分子式 : | - | |
| 纯度 : | 95% determined by reducing SDS-PAGE. | |
| 分子量 : | 69.1 KDa | |
| MDL号 : | - | |
| 存储条件： |
Pure form Sealed in dry, store in freezer, under -70°C In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : | - |
| 生物活性 | |||
|---|---|---|---|
| 描述 | Lysine-tRNA ligase, also known as Lysyl-tRNA synthetase, LysRS, KARS and KIAA0070, belongs to the class-II aminoacyl-tRNA synthetase family. The N-terminal cytoplasmic domain (1-65) is a functional tRNA-binding domain, which is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. KARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB). | ||