Metformin

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Chemical Structure| 657-24-9 同义名 : 1，1-二甲基双胍;甲福明;N,N-二甲基二胍 ;1,1-Dimethylbiguanide
CAS号 : 657-24-9
货号 : A270611
分子式 : C4H11N5
纯度 : 95%
分子量 : 129.16
MDL号 : MFCD00242652
存储条件：

Pure form Keep in dark place, inert atmosphere, room temperature

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 25 mg/mL(193.55 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 50 mg/mL(387.11 mM)，配合低频超声助溶
动物实验配方：
生物活性
描述 Metformin inhibits the mitochondrial respiratory chain in the liver, leading to activation of AMPK, enhancing insulin sensitivity for type 2 diabetes research. Metformin can cross the blood-brain barrier and triggers autophagy[2]. A short-term administration of metformin strongly protected the myocardium against isoproterenol-induced infarction[3]. Metformin decreased glycogen synthesis in a dose-dependent manner with an IC50 value of 196.5 mM. Moreover, a strong inhibition (62%) of glucose produced from lactate/pyruvate (3 mm/0.3 mm) was observed in cells treated with 350 mM metformin[4]. A potential anti-tumourigenic effect of metformin may be mediated by its role in activating AMPK, which in turn inhibits mammalian target of rapamycin (mTOR) [5]. Metformin inhibits cell growth via AMPK activation and subsequent inhibition of PI3K/AKT signaling in A-ESCs (adenomyotic endometrial stroma cells), particularly during the secretory phase, suggesting a greater effect of metformin on A-ESCs from secretory phase[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

7.74mL

1.55mL

0.77mL

38.71mL

7.74mL

3.87mL

77.42mL

15.48mL

7.74mL
参考文献

[2]Quaile MP, Melich DH, Jordan HL, Nold JB, Chism JP, Polli JW, Smith GA, Rhodes MC. Toxicity and toxicokinetics of metformin in rats. Toxicol Appl Pharmacol. 2010 Mar 15;243(3):340-7

[3]Soraya H, Khorrami A, Garjani A, Maleki-Dizaji N, Garjani A. Acute treatment with metformin improves cardiac function following isoproterenol induced myocardial infarction in rats. Pharmacol Rep. 2012;64(6):1476-84

[4]Otto M, Breinholt J, Westergaard N. Metformin inhibits glycogen synthesis and gluconeogenesis in cultured rat hepatocytes. Diabetes Obes Metab. 2003 May;5(3):189-94

[5]Mallik R, Chowdhury TA. Metformin in cancer. Diabetes Res Clin Pract. 2018 Sep;143:409-419

[6]Xue J, Zhang H, Liu W, Liu M, Shi M, Wen Z, Li C. Metformin inhibits growth of eutopic stromal cells from adenomyotic endometrium via AMPK activation and subsequent inhibition of AKT phosphorylation: a possible role in the treatment of adenomyosis. Reproduction. 2013 Aug 21;146(4):397-406