G-418 disulfate

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Chemical Structure| 108321-42-2 同义名 : 遗传霉素硫酸盐(G-418 硫酸盐) ;Geneticin sulfate; Antibiotic G-418 sulfate; G418 Sulfate; Geneticin
CAS号 : 108321-42-2
货号 : A261695
分子式 : C20H44N4O18S2
纯度 : 620u/mg
分子量 : 692.71
MDL号 : MFCD28168024
存储条件：

Pure form Keep in dark place, inert atmosphere, 2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

H2O: 120 mg/mL(173.23 mM)，配合低频超声助溶
动物实验配方：
生物活性
描述 Antibiotic G-418 is a new aminoglycoside produced by a new species of Micromonospora, M. rhodorangea NRRL 5326, with a similar structure to gentamicin B1 and has broad-spectrum antibacterial activity in vitro and in vivo, such as protozoa, amoebae, tapeworm, and pinworm infections in mice, and blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells[3]. G418 was found to kill Acanthamoeba trophozoites with IC50 and IC90 values of 32 ± 3 and 75 ± 5 μg/mL at 37 °C respectively and induced programmed cell death through the elevation of intracellular calcium and cytochrome c translocation[4]. G418 at 1000 µg/ml was shown to have considerable influence on the growth and metabolism of CHO 679 and BHK 570 cell lines, which was correlated with the copy number and expression of the resistance protein neomycin[5]. It was also found that G418 is an allosteric activator of phosphatidylinositol-specific phospholipase C (PI-PLC) in Bacillus cereus[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.44mL

0.29mL

0.14mL

7.22mL

1.44mL

0.72mL

14.44mL

2.89mL

1.44mL
参考文献

[1]Li Y, Shang P, et al. Inhibitory effects of antisense RNA of HAb18G/CD147 on invasion of hepatocellular carcinoma cells in vitro. World J Gastroenterol. 2003 Oct;9(10):2174-7.

[2]Davies J, Jimenez A. A new selective agent for eukaryotic cloning vectors. Am J Trop Med Hyg. 1980 Sep;29(5 Suppl):1089-92.

[3]Wagman GH, Testa RT, Marquez JA, Weinstein MJ. Antibiotic G-418, a new Micromonospora-produced aminoglycoside with activity against protozoa and helminths: fermentation, isolation, and preliminary characterization. Antimicrob Agents Chemother. 1974 Aug;6(2):144-9. doi: 10.1128/AAC.6.2.144. PMID: 15828184; PMCID: PMC444619.

[4]Koutsogiannis Z, MacLeod ET, Maciver SK. G418 induces programmed cell death in Acanthamoeba through the elevation of intracellular calcium and cytochrome c translocation. Parasitol Res. 2019 Feb;118(2):641-651. doi: 10.1007/s00436-018-6192-0. Epub 2019 Jan 7. PMID: 30617503; PMCID: PMC6349814.

[5]Yallop CA, Svendsen I. The effects of G418 on the growth and metabolism of recombinant mammalian cell lines. Cytotechnology. 2001 Mar;35(2):101-14. doi: 10.1023/A:1017550902771. PMID: 19003287; PMCID: PMC3449461.

[6]Morris JC, Ping-Sheng L, Zhai HX, Shen TY, Mensa-Wilmot K. Phosphatidylinositol phospholipase C is activated allosterically by the aminoglycoside G418. 2-deoxy-2-fluoro-scyllo-inositol-1-O-dodecylphosphonate and its analogs inhibit glycosylphosphatidylinositol phospholipase C. J Biol Chem. 1996 Jun 28;271(26):15468-77. doi: 10.1074/jbc.271.26.15468. PMID: 8663028.

[7]Delrue I, et al. Determination of the Selection Capacity of Antibiotics for Gene Selection. Biotechnol J. 2018 Aug;13(8):e1700747.

[8]Ye G, et al. The FAP α -activated prodrug Z-GP-DAVLBH inhibits the growth and pulmonary metastasis of osteosarcoma cells by suppressing the AXL pathway. Acta Pharm Sin B. 2022 Mar;12(3):1288-1304.

[9]Nasonovs A, et al. A549 cells contain enlarged mitochondria with independently functional clustered mtDNA nucleoids. PLoS One. 2021 Mar 25;16(3):e0249047.

[10]Heier CR, et al. Translational readthrough by the aminoglycoside geneticin (G418) modulates SMN stability in vitro and improves motor function in SMA mice in vivo. Hum Mol Genet. 2009 Apr 1;18(7):1310-22.