DOTAP chloride
产品说明书
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同义名 : | 1,2-Dioleoyl-3-trimethylammonium-propane chloride; DOTAP; 1,2-Dioleoyl-3-trimethylammoniumpropane(chloride) |
| CAS号 : | 132172-61-3 | |
| 货号 : | A227201 | |
| 分子式 : | C42H80ClNO4 | |
| 纯度 : | 98%+ | |
| 分子量 : | 698.54 | |
| MDL号 : | MFCD01310872 | |
| 存储条件： |
Pure form Inert atmosphere, store in freezer, under -20°C In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 25 mg/mL(35.79 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO 无水乙醇: 100 mg/mL(143.16 mM)，配合低频超声助溶，注意：无水乙醇开封后，易挥发，也会吸收空气中的水分，导致溶解能力下降，请避免使用开封较久的乙醇 |
| 生物活性 | |||
|---|---|---|---|
| 描述 | DOTAP chloride is a potent cationic lipid for transient and stable transfection of DNA (plasmids, baculoviruses) and modified nucleic acids (antisense oligonucleotides). DOTAP chloride recombines by binding to DNA. The transfection efficiency of DOTAP chloride is primarily affected by lipid composition and cell type, and is not affected by size or ZETA potential[1]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
1.43mL 0.29mL 0.14mL |
7.16mL 1.43mL 0.72mL |
14.32mL 2.86mL 1.43mL |
| 参考文献 |
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