2′,7′-Dichlorofluorescein diacetate

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Chemical Structure| 2044-85-1 同义名 : DCFH2-DA; 2',7'-Dichlorofluorescein diacetate; Leuco-dcf diacetate; Dcfh-DA; Diacetyldichlorofluorescein; MFCD 37501; DCFDA
CAS号 : 2044-85-1
货号 : A196793
分子式 : C24H14Cl2O7
纯度 : 98%
分子量 : 485.27
MDL号 : MFCD00037501
存储条件：

Pure form Keep in dark place,Inert atmosphere,2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 35 mg/mL(72.12 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
生物活性
描述 2′,7′-Dichlorofluorescein diacetate (DCFH2-DA) is a cell-permeable fluorescent probe. 2′,7′-Dichlorofluorescein diacetate can be used to detect the generation of reactive oxygen intermediates and for assessing the overall oxidative stress in toxicological phenomenon[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.06mL

0.41mL

0.21mL

10.30mL

2.06mL

1.03mL

20.61mL

4.12mL

2.06mL
参考文献

[1]Afri M, et al. Active oxygen chemistry within the liposomal bilayer. Part IV: Locating 2',7'-dichlorofluorescein (DCF), 2',7'-dichlorodihydrofluorescein (DCFH) and 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) in the lipid bilayer. Chem Phys Lipids. 2004 Aug;131(1):123-33.