Hoechst 33258

产品说明书

Print
Chemical Structure| 23491-44-3 同义名 : bisBenzimide H 33258; H 33258
CAS号 : 23491-44-3
货号 : A196245
分子式 : C25H24N6O
纯度 : 98%
分子量 : 424.5
MDL号 : MFCD00044760
存储条件：

Pure form Sealed in dry, store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 40 mg/mL(94.23 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Hoechst 33258 is a nuclear marker dye in Hoechst series, primarily binding to the A/T-rich regions of DNA strands. Its binding affinity is not restricted to DNA, but the fluorescence intensity is significantly enhanced when bound to A/T-rich double-stranded DNA, making it a valuable tool for live cell labeling. The fluorescence intensity of Hoechst dye increases with the solution's pH, enabling its application across a range of biological research fields[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.36mL

0.47mL

0.24mL

11.78mL

2.36mL

1.18mL

23.56mL

4.71mL

2.36mL
参考文献

[1]Wang XJ, et al. Newly synthesized bis-benzimidazole derivatives exerting anti-tumor activity through inductionof apoptosis and autophagy. Bioorg Med Chem Lett. 2012 Oct 1;22(19):6297-300.