Dihydrofluorescein diacetate
产品说明书
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同义名 : | - |
| CAS号 : | 35340-49-9 | |
| 货号 : | A192744 | |
| 分子式 : | C24H18O7 | |
| 纯度 : | 98% | |
| 分子量 : | 418.4 | |
| MDL号 : | MFCD00005056 | |
| 存储条件： |
Pure form Keep in dark place,Inert atmosphere,2-8°C In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 100 mg/mL(239.01 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
| 生物活性 | |||
|---|---|---|---|
| 描述 | Dihydrofluorescein diacetate may be an excellent fluorescent probe for many cell-based studies. It is a better fluorescent probe for detecting intracellular oxidants because it is more reactive to specific oxidants.Dihydrofluorescein diacetate exhibits fluorescence in reoxygenated endothelial cells with a linear structure consistent with mitochondria[1].Dihydrofluorescein diacetate detects the presence of ROS in mitochondria.Dihydrofluorescein diacetate fluoresces clearly, depicting thin filaments, all of which correspond in detail to TMRM-stained mitochondria. It enters the mitochondria and reacts with ROS released in the matrix[2].Dihydrofluorescein diacetate may be a useful quantitative method for measuring the oxidation potential of nanoparticle-treated cells [3]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
2.39mL 0.48mL 0.24mL |
11.95mL 2.39mL 1.20mL |
23.90mL 4.78mL 2.39mL |
| 参考文献 |
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