DMPO

产品说明书

Print
Chemical Structure| 3317-61-1 同义名 : 5,5-二甲基-1-吡咯啉-N-氧化物 ;5,5-Dimethyl-1-Pyrroline-N-Oxide
CAS号 : 3317-61-1
货号 : A189217
分子式 : C6H11NO
纯度 : 97%
分子量 : 113.16
MDL号 : MFCD00005279
存储条件：

Pure form Sealed in dry, store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(927.91 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 100 mg/mL(883.72 mM)
动物实验配方：
生物活性
描述 DMPO is a cell-permeable hydrophilic spin-trapping agent used to detect superoxide[1].Acting at a concentration of 100 μM for 24 h, DMPO attenuated SIN-1 (500 μM, 2 h) mediated cytotoxicity and ROS generation in BAEC or HEK293 cells[3].At a concentration of 100 μM for 24 h, DMPO increased NO levels by increasing eNOS activity and phosphorylated-eNOS levels in BAEC cells[3].In the concentration range of 1-100 mM, DMPO inhibited FMLP and concanavalin A-induced O2- secretion from neutrophils[4].At a concentration of 50 mM for 24 h, DMPO inhibited LPS-triggered production of M1-related pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) and NO in RAW 264.7 cells[5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

8.84mL

1.77mL

0.88mL

44.19mL

8.84mL

4.42mL

88.37mL

17.67mL

8.84mL
参考文献

[1]Ranguelova K, Mason RP. The fidelity of spin trapping with DMPO in biological systems. Magn Reson Chem. 2011;49(4):152-158.

[2]Das A, et al. Reversal of SIN-1-induced eNOS dysfunction by the spin trap, DMPO, in bovine aortic endothelial cells via eNOS phosphorylation. Br J Pharmacol. 2014 May;171(9):2321-34.

[3]Britigan BE, Hamill DR. Effect of the spin trap 5,5 dimethyl-1-pyrroline-N-oxide (DMPO) on human neutrophil function: novel inhibition of neutrophil stimulus-response coupling? Free Radic Biol Med. 1990;8(5):459-70.

[4]Zhai Z, et al. The spin trap 5,5-dimethyl-1-pyrroline N-oxide inhibits lipopolysaccharide-induced inflammatory response in RAW 264.7 cells. Life Sci. 2012 Mar 10;90(11-12):432-9.

[5]Woo suk Chung, et al. Antinociceptive Effect of Intraperitoneally Administered 5,5-dimethyl-1-pyrroline N-oxide on Formalin Induced Nociception in Rats. Korean Journal of Anesthesiology 2008;54(3):S35-S39.