SR9009

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Chemical Structure| 1379686-30-2 同义名 : REV-ERB Agonist II
CAS号 : 1379686-30-2
货号 : A183954
分子式 : C20H24ClN3O4S
纯度 : 97%
分子量 : 437.94
MDL号 : N/A
存储条件：

Pure form Sealed in dry, 2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(239.76 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 The nuclear receptors REV-ERBα and -β link circadian rhythms and metabolism. SR-9009 is a potent synthetic REV-ERB agonist with IC50 value of 710nM for suppression of transcription in a cotransfection assay using full-length REV-ERB-a along with a luciferase reporter driven by the Bmal1 promoter. Administration of SR-9009 at dose of 100mg/kg, i.p., b.i.d., for 30 days resulted in a decrease in fat mass and in plasma lipids in diet-induced obese mice. SR9009 at concentration ranging in 2.5-20μM dose-dependently decreased mESC viability, rewired cellular metabolism shown by reduction of mitochondrial respiration, both of which were independent of REV-ERBs, and altered gene transcription in hepatocytes and embryonic stem cells lacking both REV-ERBα and –β, suggesting SR9009 may not be used solely as surrogate for REV-ERB activity.
作用机制 SR-9009 directly binds to REV-ERB-α.[1]
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.28mL

0.46mL

0.23mL

11.42mL

2.28mL

1.14mL

22.83mL

4.57mL

2.28mL
参考文献

[1]Solt LA, Wang Y, et al. Regulation of circadian behaviour and metabolism by synthetic REV-ERB agonists. Nature. 2012 Mar 29;485(7396):62-8.

[2]Dierickx P, Emmett MJ, et al. SR9009 has REV-ERB-independent effects on cell proliferation and metabolism. Proc Natl Acad Sci U S A. 2019 Jun 18;116(25):12147-12152.