Ansamitocin P-3

产品说明书

Print
Chemical Structure| 66584-72-3 同义名 : Antibiotic C 15003P3; Maytansinol isobutyrate; NSC292222
CAS号 : 66584-72-3
货号 : A159572
分子式 : C32H43ClN2O9
纯度 : 98% P-3 major, mix(P-0, P-1, P-2, P-3, P-3’, P-4, P-4’)
分子量 : 635.14
MDL号 : MFCD00274586
存储条件：

Pure form Keep in dark place, inert atmosphere, store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(165.32 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
生物活性
描述 Ansamitocin P-3 (Antibiotic C 15003P3) exhibits potent anti-proliferative activity against MCF-7, HeLa, EMT-6/AR1, and MDA-MB-231 cell lines, with half-maximal inhibitory concentrations (IC50) of approximately 20±3, 50±0.5, 140±17, and 150±1.1 pM, respectively. It binds to tubulin with a dissociation constant (Kd) of about 1.3±0.7 μM, leading to tubulin's conformational changes. This compound not only significantly inhibits cell proliferation in a concentration-dependent manner but also stalls MCF-7 cell cycle progression in the G2/M phase as shown by flow cytometric analysis of propidium iodide-stained cells, with an increased percentage of cells in G2/M phase upon treatment with 50 and 100 pM Ansamitocin P3[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.57mL

0.31mL

0.16mL

7.87mL

1.57mL

0.79mL

15.74mL

3.15mL

1.57mL
参考文献

[1]Venghateri JB, et al. Ansamitocin P3 depolymerizes microtubules and induces apoptosis by binding to tubulin at thevinblastine site. PLoS One. 2013 Oct 4;8(10):e75182.