DTNB
产品说明书
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同义名 : | 埃尔曼试剂 ;Ellman’s Reagent |
| CAS号 : | 69-78-3 | |
| 货号 : | A146608 | |
| 分子式 : | C14H8N2O8S2 | |
| 纯度 : | 98% | |
| 分子量 : | 396.35 | |
| MDL号 : | MFCD00007140 | |
| 存储条件： |
Pure form Keep in dark place, sealed in dry, room temperature In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 105 mg/mL(264.92 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | DTNB, known as Ellman’s Reagent, serves as a specific chemical for quantifying the presence and concentration of thiol groups. It interacts with the free sulfhydryl side chain of cysteine, creating an S-S bond that links the protein to a thionitrobenzoic acid (TNB) residue. The primary benefits of using DTNB over other reagents, such as N-ethylmaleimide or iodoacetamide, lie in its selectivity and the capability to monitor the reaction's progress spectrophotometrically[1]. | ||
| 临床研究 | |||||
|---|---|---|---|---|---|
| NCT号 | 适应症或疾病 | 临床期 | 招募状态 | 预计完成时间 | 地点 |
| NCT01242033 | Oxidative Stress ... 展开 >> Antioxidants 收起 << | Not Applicable | Completed | - | Canada, Ontario ... 展开 >> Clinical Nutrition and Risk Factor Modification Centre; St. Michael's Hospital Toronto, Ontario, Canada, M5C 2T2 收起 << |
| NCT03230812 | Glucose Intolerance | Not Applicable | Recruiting | October 2019 | Netherlands ... 展开 >> Maastricht University Recruiting Maastricht, Limburg, Netherlands, 6229ER Contact: Vera Schrauwen, Dr. 003143874951 v.schrauwen@maastrichtuniversity.nl 收起 << |
| NCT01980212 | - | Unknown | December 2016 | China, Hunan ... 展开 >> Hunan Provincal Tumor Hospital Recruiting Changsha, Hunan, China, 410013 Contact: Nong Yang, MD +86 731 89762323 yangnong0217@163.com Contact: Chunhua Zhou, MD +86 731 89762321 zhouchunhua@hnszlyy.com Principal Investigator: Nong Yang, MD 收起 << | |
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
2.52mL 0.50mL 0.25mL |
12.62mL 2.52mL 1.26mL |
25.23mL 5.05mL 2.52mL |
| 参考文献 |
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