RN-18
产品说明书
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同义名 : | - |
| CAS号 : | 431980-38-0 | |
| 货号 : | A145644 | |
| 分子式 : | C20H16N2O4S | |
| 纯度 : | 98% | |
| 分子量 : | 380.42 | |
| MDL号 : | MFCD03142961 | |
| 存储条件： |
Pure form Sealed in dry,2-8°C In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 105 mg/mL(276.01 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | Efficient HIV-1 replication requires the expression of Vif (viral infectivity factor) protein to counter the activity of APOBEC3G (A3G), a DNA-editing cytidine deaminase expressed nonpermissive cells, which catalyzes critical hypermutations in the viral DNA and acts as an innate weapon against retroviruses. In contrast, HIV-1 replication is Vif-independent in host cells that do not express A3G (permissive cells). RN-18 is a small molecule HIV-1 Vif inhibitor with an IC50 of 6 μM in nonpermissive H9 cells. RN-18 also exhibits potent antiviral activity in the nonpermissive CEM cells with IC50 of 4.5 μM but not in permissive MT4 and CEM-SS cells [3]. In 293T cells treated with 50 μM RN-18 for 24 h, Vif protein levels were downregulated, and A3G expression increased, indicating that RN-18 inhibited Vif function and upregulated A3G levels. Similar to A3G, APOBEC3F and APOBEC3C, other members of APOBEC3 family, are also potent inhibitors of HIV-1 replication and is targeted by Vif. The levels of APOBEC3F and APOBEC3C were significantly and quantitatively enhanced in 293T cells treated with 50 μM RN-18 for 24 h [4]. | ||
| 实验方案 | |||
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| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
2.63mL 0.53mL 0.26mL |
13.14mL 2.63mL 1.31mL |
26.29mL 5.26mL 2.63mL |
| 参考文献 |
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