CHAPS
产品说明书
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同义名 : | - |
| CAS号 : | 75621-03-3 | |
| 货号 : | A137186 | |
| 分子式 : | C32H58N2O7S | |
| 纯度 : | 98% | |
| 分子量 : | 614.88 | |
| MDL号 : | MFCD00081083 | |
| 存储条件： |
Pure form Sealed in dry,2-8°C In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
H2O: 85 mg/mL(138.24 mM)，配合低频超声，并水浴加热至45℃助溶 |
| 生物活性 | |||
|---|---|---|---|
| 描述 | CHAPS is a non denaturing zwitterionic detergent for membrane biochemistry. It can be used to dissolve membrane proteins and interrupt protein-protein interactions. The zwitterionic detergent CHAPS, a derivative of the bile salts, is widely used in membrane protein solubilization. CHAPS has a low affinity for the erythrocyte membrane (partition coefficient K=0.06mM(-1)), and at sub-hemolytic concentrations it causes little effect on membrane lipid order. CHAPS hemolysis and phospholipid solubilization are closely correlated[1]. Chemical cross-linking shows that the dimerization of EnvZ-PD is significantly inhibited by the CHAPS and cholate[2]. The recovery and preservation of catalytic activity of two trans-membrane proteases, dipeptidyl peptidase IV and nephrilysin, was examined and found to be clearly superior after CHAPS treatment compared to DTT(dithiothreitol). Proteomic profiling by mass spectrometry (MS) revealed that 76.2% of proteins recovered by CHAPS are common to those seen for DTT treatment, which illustrates underlining similarities between the two approaches[3]. | ||
| 临床研究 | |||||
|---|---|---|---|---|---|
| NCT号 | 适应症或疾病 | 临床期 | 招募状态 | 预计完成时间 | 地点 |
| NCT03241810 | Metastatic Breast Cancer | Phase 2 | Recruiting | September 2020 | - |
| NCT03056755 | Breast Cancer | Phase 2 | Recruiting | April 10, 2020 | - |
| NCT03155997 | Breast Cancer | Phase 3 | Recruiting | June 10, 2027 | - |
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
|
1 mM 5 mM 10 mM |
1.63mL 0.33mL 0.16mL |
8.13mL 1.63mL 0.81mL |
16.26mL 3.25mL 1.63mL |
| 参考文献 |
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