ABTS diammonium salt

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Chemical Structure| 30931-67-0 同义名 : -
CAS号 : 30931-67-0
货号 : A1369194
分子式 : C18H24N6O6S4
纯度 : 98%
分子量 : 548.68
MDL号 : MFCD00010404
存储条件：

Pure form Keep in dark place, inert atmosphere, 2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 20 mg/mL(36.45 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 50 mg/mL(91.13 mM)
生物活性
描述 In the realm of enzyme-linked immunosorbent assay (ELISA), a micro-technique utilizing ABTS as a substrate for horseradish peroxidase (HRP) conjugate has been evaluated. Among four compared substrates—5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT), and ABTS-ABTS shines for its sensitivity, stability, and distinctive bluish-green color, enhancing its visual detectability in assays[1]. ABTS, a commonly employed peroxidase substrate, is pivotal for the large-scale cultivation and subsequent analysis of peroxidase-positive transformants, aiming at the production of active protein. Notably, after 160 hours of cultivation, an activity peak of 55,000 U/L is recorded in the presence of ABTS, highlighting the enzyme's optimal performance with this substrate. The activity notably diminishes as H2O2 concentration exceeds 0.125 mM, illustrating the enzyme's susceptibility to inhibition by H2O2, with the maximum reaction rates achieved varying based on the substrate, ranging from 31.2 to 125 μM[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.82mL

0.36mL

0.18mL

9.11mL

1.82mL

0.91mL

18.23mL

3.65mL

1.82mL
参考文献

[1]Matsuda H, et al. Evaluation of ELISA with ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med. 1984 Jun;54(3):131-8.

[2]Lauber C, et al. Identification, heterologous expression and characterization of a dye-decolorizing peroxidase of Pleurotus sapidus. AMB Express. 2017 Aug 23;7(1):164.