KM91104
产品说明书
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同义名 : | - |
| CAS号 : | 304481-60-5 | |
| 货号 : | A1249487 | |
| 分子式 : | C14H12N2O4 | |
| 纯度 : | 98% | |
| 分子量 : | 272.26 | |
| MDL号 : | MFCD00587366 | |
| 存储条件： |
Pure form Sealed in dry, 2-8°C In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 105 mg/mL(385.67 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
| 生物活性 | |||
|---|---|---|---|
| 描述 | The vacuolar H+ adenosine-triphosphatase (v-ATPase) multi-subunit complex is a key regulator of intracellular pH homeostasis. KM91104 is a cell-permeable inhibitor of v-ATPase. In hHSC (human hepatic stellate cells), KM91104 (1 nM and 10 nM; 48 hr) reduced v-ATPase expression and activity. Serum starved hHSC displayed a higher neutral red uptake compared to cells in complete medium reflecting a more acidic pH inside the lysosomes and endosomes. KM91104 (10 nM) in serum free condition reversed this effect as demonstrated by a decreased uptake of neutral red when compared to the control cells. Likewise, treatment with 10 nM KM91104 was followed by a significant decrease in cytosolic pH. Specific inhibition of v-ATPase with KM91104 induced a down-regulation of the HSC fibrogenic gene profile, which coincided with increased lysosomal pH, decreased pHi, activation of AMPK, reduced proliferation, and lower metabolic activity[3]. | ||
| 作用机制 | KM91104 specifically targets the interaction between v-ATPase subunit a3 and subunit B2[3]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
3.67mL 0.73mL 0.37mL |
18.36mL 3.67mL 1.84mL |
36.73mL 7.35mL 3.67mL |
| 参考文献 |
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