MIRA-1

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Chemical Structure| 72835-26-8 同义名 : NSC 19630
CAS号 : 72835-26-8
货号 : A1208571
分子式 : C8H9NO4
纯度 : 97%
分子量 : 183.16
MDL号 : MFCD12828765
存储条件：

Pure form Sealed in dry, 2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(573.26 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
生物活性
描述 MIRA-1 is a maleimide analogue that induces apoptosis in mutant p53 cells by restoring p53-dependent transcriptional activation. MIRA-1 inhibits the growth of Saos-2-His273 cells expressing mutant p53, and possesses anticancer activity. At a concentration of 5 μM for 14 days, MIRA-1 significantly reduced the number of colonies formed by His273-expressing Saos-2 cells, but was much less effective in inhibiting p53-deficient Saos-2 cells. MIRA-1 induced EGFP expression, MDM2 and Bax in SKOV-His175 cells at concentrations of 5 and 10 μM for 24 hours[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

5.46mL

1.09mL

0.55mL

27.30mL

5.46mL

2.73mL

54.60mL

10.92mL

5.46mL
参考文献

[1]Bykov VJ, et al. Reactivation of mutant p53 and induction of apoptosis in human tumor cells by maleimide analogs [published correction appears in J Biol Chem. 2017 Dec 1;292(48):19607]. J Biol Chem. 2005;280(34):30384-30391.