RSL3

产品说明书

Print
Chemical Structure| 1219810-16-8 同义名 : (1S,3R)-RSL3
CAS号 : 1219810-16-8
货号 : A108537
分子式 : C23H21ClN2O5
纯度 : 99%+
分子量 : 440.88
MDL号 : MFCD30187526
存储条件：

Pure form Inert atmosphere, store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(238.16 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：

IP 2% DMSO+2% Tween80+40% PEG300+water 2.4 mg/mL clear

PO 0.5% CMC-Na 28 mg/mL suspension

生物活性
描述 RSL3, also known as (1S,3R)-RSL3, acts as an inhibitor of glutathione peroxidase 4 (GPX4), which is a ferroptosis activator. It diminishes the GPX4 protein levels and prompts ferroptotic cell death in head and neck cancer cells. Additionally, RSL3 elevates the levels of p62 and Nrf2 and deactivates Keap1 in HN3-rslR cells[1]. RSL3, within concentration of 8 μM over 72 hours, significantly decreases the viability of HN3 cells. The IC50 values are 0.48 μM for HN3 cells and 5.8 μM for HN3-rslR cells, respectively. RSL3, in concentrations ranging from 0 to 8 μM over a period of 24 hours, diminishes GPX4 protein levels, augments the expression of p62 and Nrf2, and deactivates Keap1 in HN3-rslR cells[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.27mL

0.45mL

0.23mL

11.34mL

2.27mL

1.13mL

22.68mL

4.54mL

2.27mL
参考文献

[1]Shin D, et al. Nrf2 inhibition reverses resistance to GPX4 inhibitor-induced ferroptosis in head and neck cancer. Free Radic Biol Med. 2018 Dec;129:454-462.