BIS-TRIS propane

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Chemical Structure| 64431-96-5 同义名 : BTP
CAS号 : 64431-96-5
货号 : A100332
分子式 : C11H26N2O6
纯度 : 95%
分子量 : 282.33
MDL号 : MFCD00004689
存储条件：

Pure form Keep in dark place,Inert atmosphere,2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 25 mg/mL(88.55 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 100 mg/mL(354.19 mM)，配合低频超声助溶
生物活性
描述 Bis-tris propane (BTP) is a soluble buffering agent in water. It is effective as a buffer for polymerase chain reaction (PCR) processes. Additionally, bis-tris propane may improve the stability or function of restriction enzymes[1][2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.54mL

0.71mL

0.35mL

17.71mL

3.54mL

1.77mL

35.42mL

7.08mL

3.54mL
参考文献

[1]K A Eckert, et al. DNA polymerase fidelity and the polymerase chain reaction. PCR Methods Appl. 1991 Aug;1(1):17-24.

[2]J R Wenner, et al. Buffer effects on EcoRV kinetics as measured by fluorescent staining and digital imaging of plasmid cleavage. Anal Biochem.