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Trypsin/胰蛋白酶 {[allProObj[0].p_purity_real_show]}

货号:A1484002 同义名: 重组胰蛋白酶消化液 / Recombinant Trypsin; Cocoonase

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There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.

Type HazMat fee for 500 gram (Estimated)
Excepted Quantity USD 0.00
Limited Quantity USD 15-60
Inaccessible (Haz class 6.1), Domestic USD 80+
Inaccessible (Haz class 6.1), International USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic USD 100+
Accessible (Haz class 3, 4, 5 or 8), International USD 200+
Trypsin/胰蛋白酶 化学结构 CAS号:9002-07-7
Trypsin/胰蛋白酶 化学结构
CAS号:9002-07-7
Trypsin/胰蛋白酶 3D分子结构
CAS号:9002-07-7
Trypsin/胰蛋白酶 化学结构 CAS号:9002-07-7
Trypsin/胰蛋白酶 3D分子结构 CAS号:9002-07-7
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Trypsin/胰蛋白酶 纯度/质量文件 产品仅供科研

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Trypsin/胰蛋白酶 生物活性

描述 Trypsin is a serine protease enzyme that cleaves proteins at the carboxyl side of lysine or arginine residues. It activates PAR2 and PAR4 receptors and facilitates cell-to-cell membrane fusion during PDCoV infection by interacting with the S glycoprotein of PDCoV and pAPN. Additionally, trypsin promotes cell proliferation and differentiation. It finds application in wound healing and the study of neurogenic inflammation[1][2][3][4][6]. Trypsin MS grade, a serine protease enzyme, cleaves proteins on the carboxyl end of Lysine or Arginine and activates PAR2 and PAR4. It facilitates cell-to-cell membrane fusion during PDCoV infection through the interaction between PDCoV's S glycoprotein and pAPN, and enhances cell proliferation and differentiation. This enzyme is applicable in studying wound healing and neurogenic inflammation [1][2][3][4][6]. Recombinant Trypsin Solution, an animal-free enzyme, is utilized for cell or tissue digestion. It offers high stability at room temperature and performs gentle digestion, making it suitable for processing weakly adherent cells or stem cells in low serum or serum-free conditions. Unlike conventional trypsin solutions, Recombinant Trypsin Solution is devoid of animal-derived components, providing a gentle yet effective alternative for cell digestion without using animal-derived trypsin [1].

Trypsin/胰蛋白酶 动物研究

Animal study Trypsin (100-500 μg per site in 50 μL saline, intradermal injection) elicits scratching behavior in mice[5].Trypsin MS grade (100-500 μg per site in 50 μL saline, intradermal injection) triggers scratching behavior in mice [5].

Trypsin/胰蛋白酶 参考文献

[1]Bhupendra S.Kaphalia. Chapter 16 - Biomarkers of acute and chronic pancreatitis. Biomarkers in Toxicology. 2014, Pages 279-289.

[2]Yue-Lin Yang,et al. Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner. Emerg Microbes Infect. 2020 Feb 24;9(1):457-468.

[3]H Amano, et al. Trypsin promotes C6 glioma cell proliferation in serum- and growth factor-free medium. Neurosci Res. 1996 Jul;25(3):203-8.

[4]Michael J. V. White, et al. Trypsin Potentiates Human Fibrocyte Differentiation. PLoS One. 2013; 8(8): e70795.

[5]R Costa, et al. Evidence for the role of neurogenic inflammation components in trypsin-elicited scratching behaviour in mice. Br J Pharmacol. 2008 Jul;154(5):1094-103.

[6]F Schmidlin, et al. Protease-activated receptors: how proteases signal to cells. Curr Opin Pharmacol. 2001 Dec;1(6):575-82.

Trypsin/胰蛋白酶 技术信息

CAS号9002-07-7
SMILES Code NONE
MDL No. MFCD00082094
别名 重组胰蛋白酶消化液 ;Recombinant Trypsin; Cocoonase
运输蓝冰
存储条件

In solvent -20°C:3-6个月-80°C:12个月

Pure form Keep in dark place, sealed in dry, store in freezer, under -20°C

溶解方案 请根据您的动物给药指南选择适当的溶解方案。
以下溶解方案都请先按照体外实验的方式配制澄清的储备液,再依次添加助溶剂:
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议现用现配,当天使用; 以下溶剂前显示的百分比是指该溶剂在终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶
方案一
方案二
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