N-acetyl-D-Lactosamine is a disaccharide consisting of galactose and N-acetylglucose. It occurs naturally as a structural element in a variety of glycoconjugates. N-acetyl-D-Lactosamine is used to characterize lectins.
N-acetyl-D-Lactosamine is a disaccharide consisting of galactose and N-acetylglucose. It occurs naturally as a structural element in a variety of glycoconjugates. N-acetyl-D-Lactosamine is used to characterize lectins. N-Acetyl-D-lactosamine (LacNAc, Galβ1,4GlcNAc) is found in breast milk as a free disaccharide. This compound is also found as a component of human milk oligosaccharides (HMOs), which have repeating and variably branched lactose and/or LacNAc units, often attached to sialic acid and fucose monosaccharides[1]. The lectin was affected by denaturing agents such as urea (2 M: ), thiourea (2 M: ) and guanidine-HCl (0.5 M: ) and did not require Ca2+ and Mn2+ for its activity. It was a potent mitogen at 10 microg/ml towards human peripheral blood mononuclear cells with 50% growth inhibitory potential towards SiHa (human cervix ) cancer cell line at 100 microg/ml[2]. The lectins were inhibited by N-acetyl-D-lactosamine (LacNAc), a disaccharide and asialofetuin, a complex desialylated serum glycoprotein. A. intermedium showed antiproliferative effect against various human cancer cell lines in vitro[3].
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