Genistin is a isoflavone which has a role in blocking cell cycle and inducing apoptosis. It is also a modulator of estrogen receptor. Genistin can be isolated from the seeds of glycine max (L.) merr. and used as negative control of genistein.
Genistin, an isoflavone belonging to the phytoestrogen family, is a potent anti-adipogenic and anti-lipogenic agent. Proliferation of 3T3-L1 preadipocytes was unaffected by genistin and glycitein, but it was affected by 50 and 100 µM genistein and 100 µM daidzein for 48 h. Among the four isoflavones, only 50 and 100 µM genistin and genistein markedly suppressed lipid accumulation during adipogenesis in 3T3-L1 cells through a similar signaling pathway in a dose-dependent manner[3]. GS (Genistin) exhibited more cytotoxic activity against MCF-7 cells than MDA-MB-231cells. It also effectively down-modulated ER nuclear translocation as well DNA binding activity in breast cancer cells. Moreover, GS effectively induced apoptosis and suppressed levels of oncogenic markers in MCF-7 cells[4]. Genistin (5-80 μM) inhibited the contraction of IJS in a dose-dependent manner and inhibited the increased-contractility of IJS (isolated jejunal segment) induced by acetylcholine (ACh), histamine, high Ca(2+), and erythromycin, respectively. Genistin decreased myosin light chain kinase (MLCK) protein contents and MLCK mRNA expression in IJS, and inhibited both phosphorylation and Mg(2+)-ATPase activity of purified myosin[5]. Genistein was shown to arrest the growth of malignant melanoma in vitro and to inhibit ultraviolet (UV) light-induced oxidative DNA damage[6].
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