Ferrostatin-1 (Fer-1), known for its potent inhibition of ferroptosis, effectively blocks the ferroptosis triggered by Erastin in HT-1080 cells, with an EC50 value of 60 nM. As a synthetic antioxidant, Ferrostatin-1 operates through a reductive action to shield membrane lipids from damage, thus preventing cellular death. Additionally, it demonstrates antifungal properties[1][2][3]. Ferrostatin-1 thwarts the build-up of cytosolic and lipid reactive oxygen species (ROS) caused by erastin. It also protects against glutamate-triggered neurotoxicity in organotypic slices of rat brain[1]. At a concentration of 2 μM for 24 hours, Ferrostatin-1 safeguards against neurotoxicity induced by 5 mM Glutamate in rat organotypic hippocampal slice cultures (OHSC). While Ferrostatin-1 impedes lipid peroxidation, it does not affect the formation of mitochondrial ROS or the permeability of lysosomal membranes. It also prevents cell death in models of Huntington's disease (HD), periventricular leukomalacia (PVL), and kidney dysfunction[2]. At a dosage of 1 μM over 6 hours, Ferrostatin-1 hinders the oxidative degradation of unsaturated fatty acids in HT-1080 cells, leading to an increase in the count of healthy medium spiny neurons (MSNs)[3].
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