Direct Red 80/天狼猩红
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货号：A734187同义名：
Sirius Red
Direct Red 80 has been used as an ingredient in the preparation of saturated picric acid for immunohistochemical detection and quantitation of collagen in liver tissues.
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Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Direct Red 80 has been used as an ingredient in the preparation of saturated picric acid for immunohistochemical detection and quantitation of collagen in liver tissues.
Sirius Red for Collagen Staining Protocol[1] Solutions and Reagents: Solution A. Picro-sirius red Direct Red 80 ----------------------------- 0.5 g Saturated aqueous solution of picric acid ---------500 ml Add a little solid picric acid to ensure saturation (This is important). NOTE: This is a dangerous suggestion. Just add only the liquid solution.
Solution B. Acidified water Add 5 ml acetic acid (glacial) to 1 litre of water (tap or distilled).
Procedure: 1. De-wax and hydrate paraffin sections. 2. (Optional, and not usually done) Stain nuclei with Weigert's haematoxylin (as for the van Gieson method, but more strongly, then wash the slides for 10 minutes in running tap water). 3. Stain in picro-sirius red (Solution A) for one hour (This gives near-equilibrium staining, which does not increase with longer times. Shorter times should not be used, even if the colours look OK.) 4. Wash in two changes of acidified water (Solution B). 5. Physically remove most of the water from the slides by vigorous shaking or (for a few slides only) blotting with damp filter paper. 5. Dehydrate in three changes of 100% ethanol. 6. Clear in xylene and mount in a resinous medium.
Tags:
Direct Red 80 | 2610-10-8 | Sirius Red | Direct Red80 | Direct Red-80 | Neuronal Signaling | Amyloid-β | 仅供科研使用 | AmBeed-信号通路专用抑制剂
| Direct Red 80/天狼猩红 纯度/质量文件
| Direct Red 80/天狼猩红 生物活性
| Direct Red 80/天狼猩红 参考文献
| Direct Red 80/天狼猩红 实验方案
| Direct Red 80/天狼猩红 技术信息
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