BODIPY 493/503 is a lipophilic fluorescent probe used to label cellular neutral lipid contents, displaying excitation/emission maxima of 493/503 nm, respectively.
BODIPY 493/503 (BODIPY) is an alternative lipid dye with lower background staining and narrower emission spectra[2]. BODIPY 493/503 was a convenient and simple approach to visualize lipid droplets (LDs) in both sectioned skeletal muscle and cultured adult single fibers. Furthermore, the dye was effective in both fixed and nonfixed cells, and the staining seemed unaffected by permeabilization[3]. The use of the fluorescent neutral lipid dye 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503) to facilitate quantification of neutral lipid content by flow cytometry and observation of LDs (Lipid droplets) by microscopy[4]. BODIPY 493/503 contains a nonpolar structure that, upon binding to neutral lipid, emits a green fluorescence signal with a narrow wavelength range, making it an ideal fluorophore for multi-labeling experiments[5]. BODIPY 493/503 is well suited for quantitative analysis of LDs for high-throughput screening (HTS) applications[6]. BODIPY 493/503 produced an overall better staining for PHB (Poly [(R)-3-hydroxybutyric acid) than did Nile red[7]. Protocol from BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometryde novo lipogenesis[8]
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