同义名 :	CT99021 trihydrochloride; CHIR-99021 trihydrochloride; Laduviglusib trihydrochloride
	CAS号 :	1782235-14-6
	货号 :	A962469
	分子式 :	C22H21Cl5N8
	纯度 :	99%+
	分子量 :	574.72
	MDL号 :	MFCD29079432
	存储条件：	Pure form Inert atmosphere, 2-8°C In solvent -20°C:3-6个月-80°C:12个月
	溶解度 :	-
	动物实验配方：

生物活性

描述

Laduviglusib 3HCl is an effective and selective inhibitor of GSK-3α/β, with IC50 values of 10 nM and 6.7 nM, respectively, acting through competitive binding at the ATP site to inhibit GSK3α and GSK3β. It is over 500 times more selective for GSK-3 than for CDC2, ERK2, and other protein kinases. Laduviglusib 3HCl is also an effective activator of the Wnt/β-catenin signaling pathway. It enhances self-renewal in both mouse and human embryonic stem cells and induces autophagy[1][2][3]. At a concentration of 2.5 μM, Laduviglusib 3HCl treatment reduces the viability of ES-D3 cells by 24.7%, at 5 μM by 56.3%, at 7.5 μM by 61.9%, and at 10 μM by 69.2%, with an IC50 of 4.9 μM[2].

实验方案
	1mg	5mg	10mg
1 mM 5 mM 10 mM	1.74mL 0.35mL 0.17mL	8.70mL 1.74mL 0.87mL	17.40mL 3.48mL 1.74mL

参考文献
[1]Naujok O, Lentes J, et al. Cytotoxicity and activation of the Wnt/beta-catenin pathway in mouse embryonic stem cells treated with four GSK3 inhibitors. BMC Res Notes. 2014 Apr 29;7:273. [2]Ring DB, Johnson KW, et al. Selective glycogen synthase kinase 3 inhibitors potentiate insulin activation of glucose transport and utilization in vitro and in vivo. Diabetes. 2003 Mar;52(3):588-95.