同义名 :	Cyanocobalamin; Cobalamin; NSC 80365; Docigram
	CAS号 :	68-19-9
	货号 :	A884890
	分子式 :	C63H88CoN14O14P
	纯度 :	98+%
	分子量 :	1355.37
	MDL号 :	MFCD00151092
	存储条件：	Pure form Inert atmosphere, 2-8°C In solvent -20°C:3-6个月-80°C:12个月
	溶解度 :	-
	动物实验配方：

生物活性

描述

Vitamin B12 is a water soluble vitamin with a key role in the normal functioning of the brain and nervous system, and for the formation of blood. Vitamin B12 is a complex organometallic cofactor associated with three subfamilies of enzymes: the adenosylcobalamin-dependent isomerases, the methylcobalamin-dependent methyltransferases, and the dehalogenases[3]. Light excitation energy can promote covalent linkage of B12 to transcription factors with this linkage, affecting gene expression[4]. Vitamin B12 is synthesized in some bacteria but not in animals and plants. Intestinal absorption and subsequent plasma transport of vitamin B12 are mediated by specific vitamin B12-binding proteins and their receptors in mammals. Vitamin B12 taken up by the cells is enzymatically converted to coenzyme forms of vitamin B12, methyl- and adenosyl-vitamin B12, which function as coenzymes of methionine synthase (EC 2.1.13), involved in methionine biosynthesis, and methylmalonyl-CoA mutase (EC 5.4.99.2), involved in oxidation of odd-numbered fatty acids and amino acids (valine, isoleucine, and threonine), respectively[5].

实验方案
	1mg	5mg	10mg
1 mM 5 mM 10 mM	0.74mL 0.15mL 0.07mL	3.69mL 0.74mL 0.37mL	7.38mL 1.48mL 0.74mL

参考文献

[1]Moore SJ, Lawrence AD, et al. Elucidation of the anaerobic pathway for the corrin component of cobalamin (vitamin B12). Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):14906-11. [2]Banerjee R, Ragsdale SW. The many faces of vitamin B12: catalysis by cobalamin-dependent enzymes. Annu Rev Biochem. 2003;72:209-47. [3]Banerjee R, Ragsdale SW. The many faces of vitamin B12: catalysis by cobalamin-dependent enzymes. Annu Rev Biochem. 2003;72:209-247 [4]Cheng Z, Yamamoto H, Bauer CE. Cobalamin's (Vitamin B12) Surprising Function as a Photoreceptor. Trends Biochem Sci. 2016;41(8):647-650 [5]Watanabe F, Nakano Y. Nihon Rinsho. 1999;57(10):2205-2210