ML204
产品说明书
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同义名 : | CID-230710; NSC 25850; 2-piperidino-Lepidine |
| CAS号 : | 5465-86-1 | |
| 货号 : | A835423 | |
| 分子式 : | C15H18N2 | |
| 纯度 : | 99%+ | |
| 分子量 : | 226.32 | |
| MDL号 : | MFCD00817920 | |
| 存储条件： |
Pure form Sealed in dry, 2-8°C In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : | - | |
| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | Transient receptor potential canonical (TRPC) channels are Ca2+-permeable nonselective cation channels implicated in diverse physiological functions, including smooth muscle contractility and synaptic transmission[3]. ML204 is a novel, potent, and selective TRPC4 channel inhibitor with apparent IC50 values of about 1 μM in fluorescent intracellular Ca2+ assays and about 3 μM in whole-cell voltage clamp experiments[3]. ML204 blocked TRPC4β activity induced through either Gi/o stimulation by μ-opioid, 5HT1A serotonin, and M2 muscarinic receptors or Gq/11 stimulation by the endogenous M3-like muscarinic receptors. It also blocked TRPC4β channels activated by intracellularly applied GTPγS (guanosine 5'-3-O-(thio) triphosphate) or Ca2+, which bypass receptor stimulation[3]. In isolated guinea pig ileal myocytes, ML204 blocked muscarinic cation currents activated by bath application of carbachol or intracellular infusion of GTPγS, demonstrating its effectiveness on native TRPC4 currents[3]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
4.42mL 0.88mL 0.44mL |
22.09mL 4.42mL 2.21mL |
44.19mL 8.84mL 4.42mL |
| 参考文献 |
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