X-GAL

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Chemical Structure| 7240-90-6 同义名 : BCIG; 5-bromo-4-chloro-3-indolyl-β-D-Galactopyranoside
CAS号 : 7240-90-6
货号 : A788903
分子式 : C14H15BrClNO6
纯度 : 98%
分子量 : 408.63
MDL号 : MFCD00005666
存储条件:

Pure form Keep in dark place, inert atmosphere, store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月

溶解度 : -
动物实验配方:
生物活性
描述 X-GAL (BCIG) is a widely recognized chromogenic substrate for β-galactosidase, remaining colorless until enzymatic cleavage occurs. This reaction transforms X-GAL into an insoluble blue compound, allowing for easy detection. Such properties make X-GAL especially valuable in applications like blue-white screening for cloning in bacteria and assessing β-galactosidase activity across various research contexts[1].[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.45mL

0.49mL

0.24mL

12.24mL

2.45mL

1.22mL

24.47mL

4.89mL

2.45mL

参考文献

[1]Sanchez-Ramos J, et al. The X-gal caution in neural transplantation studies. Cell Transplant. 2000 Sep-Oct;9(5):657-67.

[2]Li S, et al. In-situ SERS readout strategy to improve the reliability of beta-galactosidase activity assay based on X-gal staining in shortening incubation times. Talanta. 2021 Nov 1;234:122689.