同义名 :	靛玉红-3' -单肟 ;Indirubin-3'-oxime
	CAS号 :	160807-49-8
	货号 :	A738539
	分子式 :	C16H11N3O2
	纯度 :	99%+
	分子量 :	277.28
	MDL号 :	MFCD02683594
	存储条件：	Pure form Sealed in dry, 2-8°C In solvent -20°C:3-6个月-80°C:12个月
	溶解度 :	-
	动物实验配方：

生物活性

描述

Indirubin-3'-monoxime is an antagonist to GSK-3β by competing with ATP, displaying a Ki of 0.85 μM, and a Km of 110 μM. Furthermore, it deters the phosphorylation of tau by GSK-3β, showing an IC50 roughly around 100 nM, and fully obstructs the phosphorylation at the AT100 epitope[1]. Additionally, Indirubin-3'-monoxime impedes the proliferation of vascular smooth muscle cells (VSMC) with an IC50 approximately 2 µM. It mitigates the PDGF-induced migration of VSMC and hinders the migratory response in VSMCs, while also diminishing the production of pro-migratory LT in monocytes. Indirubin-3'-monoxime equally curbs the synthesis of 5-lipoxygenase (5-LO) products in monocytes and neutrophils, with IC50 values of 5.0±1.1 and 3.7±1.2 µM, respectively, and it stands as a 5-LO inhibitor with an IC50 between 7.8-10 µM in assays free of cells[3].

实验方案
	1mg	5mg	10mg
1 mM 5 mM 10 mM	3.61mL 0.72mL 0.36mL	18.03mL 3.61mL 1.80mL	36.06mL 7.21mL 3.61mL

参考文献

[1]Leclerc S, et al. Indirubins inhibit glycogen synthase kinase-3 beta and CDK5/p25, two protein kinases involved in abnormal tau phosphorylation in Alzheimer's disease. A property common to most cyclin-dependent kinase inhibitors? J Biol Chem. 2001 Jan 5;2 [2]Sharma S, et al. Neuroprotective role of Indirubin-3'-monoxime, a GSKβ inhibitor in high fat diet induced cognitive impairment in mice. Biochem Biophys Res Commun. 2014 Oct 3;452(4):1009-15. [3]Blazevic T, et al. Indirubin-3'-monoxime exerts a dual mode of inhibition towards leukotriene-mediated vascular smooth muscle cell migration. Cardiovasc Res. 2014 Mar 1;101(3):522-32.