Direct Red 80
产品说明书
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同义名 : | Sirius Red |
| CAS号 : | 2610-10-8 | |
| 货号 : | A734187 | |
| 分子式 : | C45H26N10Na6O21S6 | |
| 纯度 : | AR | |
| 分子量 : | 1373.07 | |
| MDL号 : | MFCD00054389 | |
| 存储条件： |
Pure form Keep in dark place,Sealed in dry,2-8°C In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : | - | |
| 动物实验配方： |
| 生物活性 | |||
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| 描述 | Direct Red 80 has been used as an ingredient in the preparation of saturated picric acid for immunohistochemical detection and quantitation of collagen in liver tissues. Sirius Red for Collagen Staining Protocol[1] Solutions and Reagents: Solution A. Picro-sirius red Direct Red 80 ----------------------------- 0.5 g Saturated aqueous solution of picric acid ---------500 ml Add a little solid picric acid to ensure saturation (This is important). NOTE: This is a dangerous suggestion. Just add only the liquid solution. Solution B. Acidified water Add 5 ml acetic acid (glacial) to 1 litre of water (tap or distilled). Procedure: 1. De-wax and hydrate paraffin sections. 2. (Optional, and not usually done) Stain nuclei with Weigert's haematoxylin (as for the van Gieson method, but more strongly, then wash the slides for 10 minutes in running tap water). 3. Stain in picro-sirius red (Solution A) for one hour (This gives near-equilibrium staining, which does not increase with longer times. Shorter times should not be used, even if the colours look OK.) 4. Wash in two changes of acidified water (Solution B). 5. Physically remove most of the water from the slides by vigorous shaking or (for a few slides only) blotting with damp filter paper. 5. Dehydrate in three changes of 100% ethanol. 6. Clear in xylene and mount in a resinous medium. |
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| 实验方案 | |||
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| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
0.73mL 0.15mL 0.07mL |
3.64mL 0.73mL 0.36mL |
7.28mL 1.46mL 0.73mL |
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