同义名 :	TSA TFA; Tubastatin A (trifluoroacetate salt); Tubastatin A trifluoroacetate salt
	CAS号 :	1239262-52-2
	货号 :	A531870
	分子式 :	C22H22F3N3O4
	纯度 :	99%+
	分子量 :	449.42
	MDL号 :	MFCD24873137
	存储条件：	Pure form Inert atmosphere, 2-8°C In solvent -20°C:3-6个月-80°C:12个月
	溶解度 :	-
	动物实验配方：

生物活性

描述

Tubastatin A exhibits significant selectivity towards all 11 HDAC isoforms, maintaining over 1000-fold selectivity against all isoforms except HDAC8, for which it demonstrates approximately 57-fold selectivity. In assays assessing homocysteic acid (HCA)-induced neurodegeneration, Tubastatin A demonstrates dose-dependent protection against HCA-induced neuronal cell death, with significant protection observed at 5 μM and near-complete protection at 10 μM[1]. Treatment of CC12 cells with Tubastatin A results in impaired myotube formation when alpha-tubulin undergoes early hyperacetylation during the myogenic process; however, myotube elongation occurs when alpha-tubulin is hyperacetylated in mature myotubes[3]. A recent study suggests that treatment with Tubastatin A enhances cell elasticity, as demonstrated by atomic force microscopy (AFM) tests, without causing significant alterations to the actin microfilament or microtubule networks in mouse ovarian cancer cell lines, MOSE-E and MOSE-L[4].

实验方案
	1mg	5mg	10mg
1 mM 5 mM 10 mM	2.23mL 0.45mL 0.22mL	11.13mL 2.23mL 1.11mL	22.25mL 4.45mL 2.23mL

参考文献

[1]de Zoeten EF, et al. Histone deacetylase 6 and heat shock protein 90 control the functions of Foxp3(+) T-regulatory cells. Mol Cell Biol. 2011 May;31(10):2066-78. [2]Kyle V. Butler et al. Rational Design and Simple Chemistry Yield a Superior, Neuroprotective HDAC6 Inhibitor, Tubastatin A J. Am. Chem. Soc., 2010, 132 (31), pp 10842-10846 [3]Di Fulvio S, et al. Dysferlin interacts with histone deacetylase 6 and increases alpha-tubulin acetylation. PLoS One. 2011;6(12):e28563 [4]Ketene AN, et al. Actin filaments play a primary role for structural integrity and viscoelastic response in cells. Integr Biol (Camb). 2012 May;4(5):540-9.