ID-8

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Chemical Structure| 147591-46-6 同义名 : -
CAS号 : 147591-46-6
货号 : A512614
分子式 : C16H14N2O4
纯度 : 99%+
分子量 : 298.29
MDL号 : MFCD00593367
存储条件：

Pure form Sealed in dry, 2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 : -
动物实验配方：
生物活性
靶点

  • DYRK

  • DYRK
描述 The dual-specificity tyrosine-phosphorylation-regulated (DYRK) kinases belong to a family collectively referred to as CMGC kinases that includes cyclin dependent kinases, mitogen associated protein kinases, glycogen synthase kinases, and cyclin dependent-like kinases. ID-8, a DYRK inhibitor, shows high specificity for DYRK1A and DYRK1B with IC50s of 78 and 54 nM, respectively[3]. ID-8 increased hESC (human embryonic stem cells) survival (∼1.1% at 0.5 μM). The combination of Wnt3a and ID-8 further increased survival (∼1.7%) and completely prevented Wnt-induced differentiation morphologically without disrupting proliferation. Wnt-induced differentiation marker gene expression was dramatically reduced by ID-8 at a dose that maintained the undifferentiated state[4]. Treatment with ID-8 (10 µM) promoted significant increases in cell number after 24 hours of damage in at least two different damage models[5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.35mL

0.67mL

0.34mL

16.76mL

3.35mL

1.68mL

33.52mL

6.70mL

3.35mL
参考文献

[1]Hasegawa K, Yasuda SY, et al. Wnt signaling orchestration with a small molecule DYRK inhibitor provides long-term xeno-free human pluripotent cell expansion. Stem Cells Transl Med. 2012 Jan;1(1):18-28.

[2]Miyabayashi T, Yamamoto M, et al. Indole derivatives sustain embryonic stem cell self-renewal in long-term culture. Biosci Biotechnol Biochem. 2008 May;72(5):1242-8.

[3]Bellmaine SF, Ovchinnikov DA, Manallack DT, et al. Inhibition of DYRK1A disrupts neural lineage specificationin human pluripotent stem cells. Elife. 2017;6:e24502

[4]Hasegawa K, Yasuda SY, Teo JL, et al. Wnt signaling orchestration with a small molecule DYRK inhibitor provides long-term xeno-free human pluripotent cell expansion. Stem Cells Transl Med. 2012;1(1):18-28

[5]Monteiro MB, Ramm S, Chandrasekaran V, et al. A High-Throughput Screen Identifies DYRK1A Inhibitor ID-8 that Stimulates Human Kidney Tubular Epithelial Cell Proliferation. J Am Soc Nephrol. 2018;29(12):2820-2833