5-TAMRA
产品说明书
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同义名 : | 5-Carboxytetramethylrhodamine |
| CAS号 : | 91809-66-4 | |
| 货号 : | A505764 | |
| 分子式 : | C25H22N2O5 | |
| 纯度 : | 99%+ | |
| 分子量 : | 430.45 | |
| MDL号 : | MFCD00269769 | |
| 存储条件： |
Pure form Keep in dark place, inert atmosphere, room temperature In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : | - | |
| 动物实验配方： |
| 生物活性 | |||
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| 描述 | 5-TAMRA (5-Carboxytetramethylrhodamine), SE and its conjugates yield bright, pH-insensitive orange-red fluorescence (approximate excitation/emission maxima ~546/579) with good photostability. 5-TAMRA is a purified monomer. It is widely used as a marker of peptides and proteins. In some complex biological studies, the reproducibility of experiments is more critical than material consumption, and it has become the preferred fluorescent labeling reagent. There is only slight difference between 5-TAMRA and 6-tamra in structure, but it will significantly affect the biological properties of the conjugate formed. TEMPO-5-TAMRA fluorescence quenching is a promising method to measure short distance changes within single biomolecules. In combination with distance calculations using molecular mechanics modeling, the experimental results from steady-state ensemble fluorescence and fluorescence correlation spectroscopy measurements both show an exponential decrease in the quenching rate constant with the dye-quencher distance in the 10-30 A range[3]. | ||
| 实验方案 | |||
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| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
2.32mL 0.46mL 0.23mL |
11.62mL 2.32mL 1.16mL |
23.23mL 4.65mL 2.32mL |
| 参考文献 |
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