同义名 :	-
	CAS号 :	1208315-24-5
	货号 :	A502981
	分子式 :	C24H22N4O3
	纯度 :	99%+
	分子量 :	414.46
	MDL号 :	MFCD22123247
	存储条件：	Pure form Sealed in dry, 2-8°C In solvent -20°C:3-6个月-80°C:12个月
	溶解度 :	-
	动物实验配方：

生物活性
描述	S-Nitrosoglutathione reductase (GSNOR) is a zinc-dependent, NAD+- and NADH-dependent, medium chain alcohol dehydrogenase (ADH) that plays an important role in nitrosative biology by metabolizing the S-nitrosothiol, GSNO. N6022 is a potent, selective and reversible GSNOR inhibitor with an IC50 of 8 nM and a Ki of 2.5 nM[3]. In cytochrome P450 assays, N6022 potently inhibited CYP2C19 with an IC50 of 0.77 μM. And it exhibited minimal cytotoxicity (IC50 > 100 μM) toward A549 epithelial lung cells. In a mouse model of ovalbumin-induced asthma, N6022 attenuated methacholine-induced broncho-constriction (airway hyperresponsiveness) in a dose- and time-dependent manner, with significant efficacy achieved with a single IV dose ≥0.01 mg/kg[4].
作用机制	N6022 binds in the GSNO substrate binding pocket like a competitive inhibitor[3].

实验方案
	1mg	5mg	10mg
1 mM 5 mM 10 mM	2.41mL 0.48mL 0.24mL	12.06mL 2.41mL 1.21mL	24.13mL 4.83mL 2.41mL

参考文献

[1]Green LS, Chun LE, et al. Mechanism of inhibition for N6022, a first-in-class drug targeting S-nitrosoglutathione reductase. Biochemistry. 2012 Mar 13;51(10):2157-68. [2]Sun X, Wasley JW, et al. Discovery of s-nitrosoglutathione reductase inhibitors: potential agents for the treatment of asthma and other inflammatory diseases. ACS Med Chem Lett. 2011 Mar 11;2(5):402-6. [3]Green LS, Chun LE, et al. Mechanism of inhibition for N6022, a first-in-class drug targeting S-nitrosoglutathione reductase. Biochemistry. 2012 Mar 13;51(10):2157-68 [4]Sun X, Wasley JW, et al. Discovery of s-nitrosoglutathione reductase inhibitors: potential agents for the treatment of asthma and other inflammatory diseases. ACS Med Chem Lett. 2011 Mar 11;2(5):402-6