Kartogenin
产品说明书
 |
同义名 : | KGN |
| CAS号 : | 4727-31-5 | |
| 货号 : | A305493 | |
| 分子式 : | C20H15NO3 | |
| 纯度 : | 98% | |
| 分子量 : | 317.34 | |
| MDL号 : | MFCD00560510 | |
| 存储条件： |
Pure form Sealed in dry, room temperature In solvent -20°C:3-6个月-80°C:12个月 |
|
| 溶解度 : | - | |
| 动物实验配方： |
2% DMSO+40% PEG 300+2% Tween80+water 5 mg/mL |
| 生物活性 | |||
|---|---|---|---|
| 描述 | Kartogenin (KGN) is a small molecule that promotes the selective differentiation of mesenchymal stem cells into chondrocytes, and thus, KGN stimulates cartilage regeneration. KGN also possess chondro-protective effect. Kartogenin (100 nM; 72 h) induces chondrocyte nodule formation in primary hMSCs. Kartogenin (10 nM-10 μM; 72 h) increases chondrocyte-specific gene expression in hMSCs. Kartogenin (0.12-10 μM; 48 h) inhibits nitric oxide (NO) and glycosaminoglycan (GAG) release induced by cytokines in primary bovine articular chondrocytes. Kartogenin (10 μM in 4μL of saline; i.a. on days 7 and 21) promotes cartilag erepair in collagenase VII-induced OA models in mice[3]. Kartogenin binds filamin A, disrupts its interaction with the transcription factor core-binding factor β subunit (CBFβ), and induces chondrogenesis by regulating the CBFβ-RUNX1 transcriptional program[4]. The DMM (destabilization of the medial meniscus) animal model showed increased articular cartilage thickness after IA (intra-articular) KGN injection. IHC staining also demonstrated upregulation of Stat3 phosphorylation and enhanced distribution of CD44+/CD105+ cells in cartilage following IA KGN injection[5]. KGN preconditioning likely improves the chondrogenic differentiation of hUCMSCs (human umbilical cord MSCs) by committing them to a precartilaginous stage with enhanced JNK phosphorylation and suppressed β-catenin[6]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
|
1 mM 5 mM 10 mM |
3.15mL 0.63mL 0.32mL |
15.76mL 3.15mL 1.58mL |
31.51mL 6.30mL 3.15mL |
| 参考文献 |
|---|