同义名 :	Bis(choline)tetrathiomolybdate; ALXN1840; Decuprate; Bis-choline tetrathiomolybdate
	CAS号 :	649749-10-0
	货号 :	A252851
	分子式 :	C10H28MoN2O2S4
	纯度 :	98%+
	分子量 :	432.54
	MDL号 :	MFCD25976790
	存储条件：	Pure form Sealed in dry,2-8°C In solvent -20°C:3-6个月-80°C:12个月
	溶解度 :	-
	动物实验配方：

生物活性

描述

ATN-224 demonstrates a unique and robust attraction to copper ions (108 mol/L), without affinity for calcium, iron, magnesium, zinc, or manganese ions up to concentrations of 1 mM, as revealed by isothermal titration calorimetry. It restricts the growth of HUVEC (IC50=1.4±0.3 μM; n=5) and inhibits bovine SOD1 activity (IC50=0.33±0.03 μM after 24 hours). The suppression of SOD1 by ATN-224 is both time-bound, peaking around 16 hours, and dose-dependent, demonstrating an IC50 of 17.5±3.7 nM in endothelial cells. Additionally, it deters FGF-2-stimulated ERK1/2 phosphorylation correlating with its anti-proliferative IC50 range (1.25-2.5 μM)[1]. Orally administered ATN-224 targets and inhibits the copper/zinc-dependent superoxide dismutase 1 (Cu/Zn-SOD1) in endothelial and cancer cells[2].

实验方案
	1mg	5mg	10mg
1 mM 5 mM 10 mM	2.31mL 0.46mL 0.23mL	11.56mL 2.31mL 1.16mL	23.12mL 4.62mL 2.31mL

参考文献

[1]Juarez JC, et al. Copper binding by tetrathiomolybdate attenuates angiogenesis and tumor cell proliferation through the inhibition of superoxide dismutase 1. Clin Cancer Res. 2006 Aug 15;12(16):4974-82. [2]Lin J, et al. A non-comparative randomized phase II study of 2 doses of ATN-224, a copper/zinc superoxide dismutase inhibitor, in patients with biochemically recurrent hormone-na?ve prostate cancer. Urol Oncol. 2013 Jul;31(5):581-8.