同义名 :	-
	CAS号 :	2093393-05-4
	货号 :	A157358
	分子式 :	C17H16N4O5S
	纯度 :	98%+
	分子量 :	388.4
	MDL号 :	N/A
	存储条件：	Pure form Inert atmosphere, 2-8°C In solvent -20°C:3-6个月-80°C:12个月
	溶解度 :	-
	动物实验配方：

生物活性
描述	Mixed lineage kinase domain-like protein (MLKL) is a necroptosis executor that mediates ion influx and causes cell structure disruption. TC13172 is a MLKL inhibitor that shows anti-necroptosis potency with an EC50 value of 2  0.6 nM in TNF-a/Smac mimetic/Z-VAD-fmk-treated HT-29 cells. In HT-29 cells pre-incubated with 100 nM TC13172 for 2 hours and then treated with another MLKL inhibitor with a propyne group at the dose of 1 μM for additional 2 hours, TC13172 efficiently outcompeted the other inhibitor in binding to MLKL. Treatment of HT-29 cells with 100 nM TC13172 for 8 hours blocked the oligomerization and translocation of MLKL from the cytoplasm to the cell membrane but did not disrupt the phosphorylation of MLKL[2].
作用机制	TC13172 potently inhibits MLKL by directly binding to Cys-86. The hydrogen bond formation between the 3-OH of TC13172 and an acceptor residue on MLKL may contribute to the high potency of TC13172 towards MLKL.

实验方案
	1mg	5mg	10mg
1 mM 5 mM 10 mM	2.57mL 0.51mL 0.26mL	12.87mL 2.57mL 1.29mL	25.75mL 5.15mL 2.57mL

参考文献

[1]Discovery of a new class of highly potent necroptosis inhibitors targeting the mixed lineage kinase domain-like protein. [2]Yan B, Liu L, Huang S, Ren Y, Wang H, Yao Z, Li L, Chen S, Wang X, Zhang Z. Discovery of a new class of highly potent necroptosis inhibitors targeting the mixed lineage kinase domain-like protein. Chem Commun (Camb). 2017 Mar 28;53(26):3637-3640. doi: 10.1039/c7cc00667e. PMID: 28267172.