NM107
产品说明书
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同义名 : | 2'-C-Methylcytidine; 2CMC; mCyd; 2'-C-MeC |
| CAS号 : | 20724-73-6 | |
| 货号 : | A147052 | |
| 分子式 : | C10H15N3O5 | |
| 纯度 : | 98% | |
| 分子量 : | 257.24 | |
| MDL号 : | MFCD02682947 | |
| 存储条件： |
Pure form Keep in dark place, inert atmosphere, store in freezer, under -20°C In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : | - | |
| 动物实验配方： |
| 生物活性 | |||
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| 描述 | Hepatitis C virus (HCV) is a 9.6-kb positive-sense, single-stranded RNA virus. The NS5B RNA-dependent RNA polymerase has enzymatic activity essential for viral replication. NM107 is a nucleoside HCV NS5B polymerase inhibitor. In HCV replicon cells (Huh-luc/neo-ET), treatment with NM107 for 48 h resulted in a concentration-dependent inhibition of HCV replication, the EC50 and CC50 were 0.48 and ＞ 100 μM, respectively. The combination of 2 μM NM107 and 0.5 μM NIM811 (a host cyclophilin inhibitor) led to a 2.1-log reduction. Furthermore, the combination of 0.5 μM NIM811 and 5 μM NM107 resulted in a 3.1-log HCV RNA reduction, which was much better than either used alone[3]. In Huh7 cultures, treatment of infected cells with 25 μM NM107 for 24 h resulted in an intracellular HCV RNA decline, consistent with NM107 blocking HCV replication. Moreover, extracellular virus titers declined in parallel with intracellular RNA levels as expected[4]. | ||
| 实验方案 | |||
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| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
3.89mL 0.78mL 0.39mL |
19.44mL 3.89mL 1.94mL |
38.87mL 7.77mL 3.89mL |
| 参考文献 |
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