同义名 :	JAK Inhibitor I; CMP 6; Janus-Associated Kinase Inhibitor I; Compound 6
	CAS号 :	457081-03-7
	货号 :	A143706
	分子式 :	C18H16FN3O
	纯度 :	98%
	分子量 :	309.34
	MDL号 :	MFCD06411425
	存储条件：	Pure form Sealed in dry, 2-8°C In solvent -20°C:3-6个月-80°C:12个月
	溶解度 :	-
	动物实验配方：

生物活性

描述

Pyridone 6 inhibits a range of 21 protein kinases with IC50s varying from 130 nM to over 10 µM. It effectively blocks IL2-driven and IL4-driven proliferation of CTLL cells with IC50s of 0.1 µM and 0.052 µM, respectively[1]. Pyridone 6 (P6) interacts with the ATP-binding cleft of JAK kinases, showing an IC50 of 3 nM for various cytokines. It strongly inhibits Th2 cell development and moderately inhibits Th1, while promoting Th17 differentiation within certain concentration ranges. P6 reduces IFN-γ and IL-13 levels but increases IL-17 and IL-22 expression, indicating its role in promoting Th17 cell differentiation from naïve T cells[2].

实验方案
	1mg	5mg	10mg
1 mM 5 mM 10 mM	3.23mL 0.65mL 0.32mL	16.16mL 3.23mL 1.62mL	32.33mL 6.47mL 3.23mL

参考文献
[1]Thompson JE, et al. Photochemical preparation of a pyridone containing tetracycle: a Jak protein kinase inhibitor. Bioorg Med Chem Lett. 2002 Apr 22;12(8):1219-23. [2]Nakagawa R, et al. Pyridone 6, a pan-JAK inhibitor, ameliorates allergic skin inflammation of NC/Nga mice via suppression of Th2 and enhancement of Th17. J Immunol. 2011 Nov 1;187(9):4611-20.