MID-1
产品说明书
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同义名 : | - |
| CAS号 : | 312608-54-1 | |
| 货号 : | A1371444 | |
| 分子式 : | C12H11N3O4S | |
| 纯度 : | 99%+ | |
| 分子量 : | 293.3 | |
| MDL号 : | MFCD01216148 | |
| 存储条件： |
Pure form Sealed in dry, 2-8°C In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : | - | |
| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | MID-1 is a MG53-IRS-1 interaction disruptor. It abolished MG53-mediated IRS-1 ubiquitination and degradation, as well as sensitized insulin signaling and increased insulin-elicited glucose uptake with an elevated level of IRS-1 in C2C12 myotubes. MID-1 (5 μM; 24 h) increases the IRS-1 expression level in skeletal muscle by disrupting the MG53-IRS-1 interaction. MID-1 (10 μM; 12 h) reduces the luciferase activity in HEK 293 cell line expressing NLUC-IRS-1 and CLUC-C14A. MID-1 (1-20 μM; 12 h) disrupts the MG53-IRS-1 interaction but not MG53-FAK interaction in HEK 293 cells. MID-1 (0.1-10 μM; 4-24 h) abolishes MG53-induced IRS-1 ubiquitination and degradation in HEK 293 cells. MID-1 (5-10 μM; 24 h) increases insulin signaling and insulin-elicited glucose uptake in C2C12 myotubes. MID-1 (5-10 μM; 24 h) enhances skeletal myogenesis[2]. NCS-1 (neuronal calcium sensor-1) binds to the Ca2+ permeable channel MID-1 for tolerance to Ca2+ stress. CRZ-1 binds to a unique sequence in the ncs-1 promoter, causing upregulation of NCS-1 that binds to MID-1 for tolerance to Ca2+ stress[3]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
3.41mL 0.68mL 0.34mL |
17.05mL 3.41mL 1.70mL |
34.09mL 6.82mL 3.41mL |
| 参考文献 |
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