同义名 :	SAG hydrochloride
	CAS号 :	2095432-58-7
	货号 :	A125368
	分子式 :	C28H29Cl2N3OS
	纯度 :	99%+
	分子量 :	526.52
	MDL号 :	N/A
	存储条件：	Pure form Inert atmosphere, 2-8°C In solvent -20°C:3-6个月-80°C:12个月
	溶解度 :	-
	动物实验配方：

生物活性
描述	SAG hydrochloride is a highly effective agonist of the Smoothened (Smo) receptor (EC50=3 nM; KD=59 nM). It activates the Hedgehog signaling pathway and antagonizes the inhibitory effects of Cyclopamine on Smo[1][2][3].

细胞研究
细胞系	浓度	检测类型	检测时间	活性说明	数据源
HUVEC		Cytotoxicity assay	24 h	Cytotoxicity against HUVEC after 24 hrs by WST-1 assay, IC50=22 μM	22985958
mouse Shh-Light II cell		Function assay	48 hrs	Activation of Shh pathway in mouse Shh-Light II cells assessed as induction of gli1 expression after 48 hrs by renilla luminescence assay, EC50=0.0088 μM	22985958

实验方案
	1mg	5mg	10mg
1 mM 5 mM 10 mM	1.90mL 0.38mL 0.19mL	9.50mL 1.90mL 0.95mL	18.99mL 3.80mL 1.90mL

参考文献

[1]Chen JK, et al. Small molecule modulation of Smoothened activity. Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14071-6. [2]Stanton BZ, et al. A small molecule that binds Hedgehog and blocks its signaling in human cells. Nat Chem Biol. 2009 Mar;5(3):154-6. [3]Guerrini G, et, al. Inhibition of smoothened in breast cancer cells reduces CAXII expression and cell migration. J Cell Physiol. 2018 Dec; 233(12): 9799-9811. [4]Lee S, et al. Combining Smoothened Agonist (SAG) and NEL-like protein-1 (NELL-1) Enhances Bone Healing. Plast Reconstr Surg. 2017 Feb 13.