同义名 :	ML249; CID-17253208
	CAS号 :	945128-26-7
	货号 :	A108628
	分子式 :	C19H28N2O2
	纯度 :	99%+
	分子量 :	316.44
	MDL号 :	MFCD09782785
	存储条件：	Pure form Sealed in dry, 2-8°C In solvent -20°C:3-6个月-80°C:12个月
	溶解度 :	-
	动物实验配方：

生物活性
描述	Sphingosine 1-phosphate 3 (S1P3) receptor is distributed on cardiac myocytes and cardiac fibroblasts in vivo and it plays a critical role in cardiovascular physiology, especially pace-making activity. CYM 5541 is a full S1P3-selective agonist, able to reach the maximum level of ERK phosphorylation that was observed with S1P. CYM-5541 has an EC50 of between 72 and 132 nM and exhibits exquisite selectivity over other S1P receptor subtypes in vitro: S1P1 EC50 > 10 μM, S1P2 EC50 > 50 μM, S1P4 EC50 > 50 μM, and S1P5 EC50 > 25 μM[3]. 10 nM of CYM5541 promotes the proliferation and attenuates apoptosis of bone marrow (BM)-derived Endothelial progenitor cells induced by H2O2Wang H, Huang H, Ding SF. Sphingosine-1-phosphate promotes the proliferation and attenuates apoptosis of Endothelial progenitor cells via S1PR1/S1PR3/PI3K/Akt pathway. Cell Biol Int. 2018 Nov;42(11):1492-1502. doi: 10.1002/cbin.10991.
作用机制	CYM 5541 sits in the hydrophobic pocket close to F263[3].

实验方案
	1mg	5mg	10mg
1 mM 5 mM 10 mM	3.16mL 0.63mL 0.32mL	15.80mL 3.16mL 1.58mL	31.60mL 6.32mL 3.16mL

参考文献

[1]Jo E, Bhhatarai B, et al. Novel selective allosteric and bitopic ligands for the S1P(3) receptor. ACS Chem Biol. 2012 Dec 21;7(12):1975-83. [2]Guerrero M, Poddutoori R, et al. Probe Development Efforts for an Allosteric Agonist of the Sphingosine 1-phosphate Receptor 3 (S1P3). 2011 Aug 31 [updated 2013 Feb 25] . Probe Reports from the NIH Molecular Libraries Program [Internet] . [3]Jo E, Bhhatarai B, Repetto E, et al. Novel selective allosteric and bitopic ligands for the S1P(3) receptor. ACS Chem Biol. 2012 Dec 21;7(12):1975-83