Arg-Gly-Asp-Ser

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Chemical Structure| 91037-65-9 同义名 : RGDS peptide; Fibronectin tetrapeptide; H-Arg-Gly-Asp-Ser-OH; Fibronectin Inhibitor
CAS号 : 91037-65-9
货号 : A107716
分子式 : C15H27N7O8
纯度 : 99%+
分子量 : 433.42
MDL号 : MFCD00076452
存储条件：

Pure form Keep in dark place, inert atmosphere, store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 : -
动物实验配方：
生物活性
描述 Integrins mediate cell-cell and cell-extracellular matrix (ECM) interactions and recognize several ECM proteins. One of the major sites mediating integrin action is the Arg-GlyAsp (RGD) motif, present in ECM proteins and in other proteins such as disintegrins. Arg-Gly-Asp-Ser (RGDS) is a synthetic integrin antagonist, a specific peptide motif through which extracellular matrix (ECM) components interact with integrin and can modify the behavior of cells. Pretreatment with RGDS at 2.5 or 5 mg/kg, 1h before LPS, inhibited LPS-induced increases in neutrophil and macrophage numbers, total protein levels and TNF-α and MIP-2 levels, and matrix metalloproteinase-9 activity in bronchoalveolar lavage (BAL) fluid at 4 or 24h post-LPS treatment. RGDS also inhibited LPS-induced phosphorylation of focal adhesion kinase and MAP kinases, including ERK, JNK, and p38 MAP kinase, in lung tissue[3]. Osteoblast-like MC3T3-E1 cells grown on the RGDS(1 and 5 mM) substrate and treated with 0.1 and 0.5 μM staurosporine showed no significant cell killing which was mediated by phosphatidylinositol 3-kinase[4]. RGDS at 500 μg/mL exhibited a dose-dependent inhibitory effect on human umbilical vein endothelial cells (HUVECs) adhesion to fibronectin and vitronectin (61% ± 4.3% and 84% ± 6.8% inhibition) and significantly reduced FGF-2–induced proliferation (50% ± 4% inhibition) in a concentration-dependent way. It was found that RGDS treatment induced caspase 8 and caspase 9 activation (1.8 ± 0.02 fold and 2.2 ± 0.2 fold versus control, respectively), while it had no effect on caspase 1 and caspase 3/7 activity[5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.31mL

0.46mL

0.23mL

11.54mL

2.31mL

1.15mL

23.07mL

4.61mL

2.31mL
参考文献

[1]Moon C, Han JR, et al. Synthetic RGDS peptide attenuates lipopolysaccharide-induced pulmonary inflammation by inhibiting integrin signaled MAP kinase pathways. Respir Res. 2009 Mar 9;10:18.

[2]Grigoriou V, Shapiro IM, et al. Apoptosis and survival of osteoblast-like cells are regulated by surface attachment. J Biol Chem. 2005 Jan 21;280(3):1733-9. Epub 2004 Nov 1.

[3]Moon C, Han JR, Park HJ, Hah JS, Kang JL. Synthetic RGDS peptide attenuates lipopolysaccharide-induced pulmonary inflammation by inhibiting integrin signaled MAP kinase pathways. Respir Res. 2009 Mar 9;10(1):18. doi: 10.1186/1465-9921-10-18. PMID: 19272161; PMCID: PMC2666640.

[4]Grigoriou V, Shapiro IM, Cavalcanti-Adam EA, Composto RJ, Ducheyne P, Adams CS. Apoptosis and survival of osteoblast-like cells are regulated by surface attachment. J Biol Chem. 2005 Jan 21;280(3):1733-9. doi: 10.1074/jbc.M402550200. Epub 2004 Nov 1. PMID: 15522882.

[5]Aguzzi MS, Giampietri C, De Marchis F, Padula F, Gaeta R, Ragone G, Capogrossi MC, Facchiano A. RGDS peptide induces caspase 8 and caspase 9 activation in human endothelial cells. Blood. 2004 Jun 1;103(11):4180-7. doi: 10.1182/blood-2003-06-2144. Epub 2004 Feb 24. PMID: 14982875.